Dongmei Hou, Ph.D., M.D

Staff Research Associate

Punch Worthington Research Laboratory
Center for the Health Sciences, Room 64-123
David Geffen School of Medicine
10833 Le Conte Avenue
Los Angeles, California 90095-1741

Phone: (310) 206-7015
E-mail: dongmeih@ucla.edu

Education

Professional Experience

 Expertise

Physiology: Patch-Clamp technique, Cut-Open Oocyte Vaseline Gap voltage clamp intracellular recording, extracellular recording, data analysis by using various soft wares

Developmental Biology: Immunohistochemistry, Immunofluorescence, Confocal microscopy, Deconvolution microscopy, in situ hybridization, cell culture, make a new construct.

Embryology:  Micromanipulation, Embryo collection and culture, Immunosurgery

Molecular Biology: Recombinant DNA, PCR and RT-PCR, Sequencing, in vitro transcription, Northern blot

Cell Biology: Primary cell culture, Cancer cell culture, Subculture, Transfection, Proliferation assay, Apoptosis assay

Biochemistry: Western blot, Two-dimension gel, Isoelectric-focusing gel, Immunoprecipitation

Surgery: Rehabilitation surgery, Facial Plastic Surgery, Micro vascular Surgery, Laryngology, and Neuro-otology

Journal Publications

1. Dongmei Hou, Kazuhiro Suzuki, William J. Wolfgang, Catherine Clay, Michael Forte, and Yoshiaki Kidokoro (2003)   Presynaptic Impairment of Synaptic Transmission in    Drosophila Embryos Lacking Gsα.    The Journal of Neuroscience 23(13):5897-5905.

2. Masaki Sone, Emiko Suzuki, Mikio Hoshino, Dongmei Hou, Hiroshi Kuromi, Masaki
Fukata, Shinya Kuroda, Kozo Kaibuchi, Yo-ichi Nabeshima and Chihiro Hama (2000) Synaptic development is controlled in the periactive zones of Drosophila synapses. Development 127, 4157-4168

3. Dongmei Hou, Jingwei Qi (1995)  Clinical Study of 118 Pediatric laryngitis.  Journal of Clinical Otorhinolaryngology (Now named as Journal of Clinical Otorhinolaryngology Head and Neck Surgery) 9(12), 13-14

4.  Jingwei Qi, Dongmei Hou (1994)  The Effects of Fiblaryngoscope in the Diagnosis and Treatment of Laryngeal Disease.  Dalian Medical College Journal of Medicine (Now named as Dalian Medical Journal) 16(4)286-288

5. Daniel Sigg, Dongmei Hou, Riccardo Olcese  (2007)  Permeation in Large and Small Conductance K+ Channels Probed by Temperature and Viscosity Sensitivity.  Submitted to Biophysical Journal. Daniel Sigg and Dongmei Hou contributed equally to this work.

Book Chapters

1. Dongmei Hou (2002) “Frozen Cosmetology” in “21st Century Medical University Teaching: Physical Therapy and Gymnastic Cosmetology” Zaien Hou, Jingwei Qi and Ming Guo, eds. Science Press China (ISBN: 7-03-010652-0/R.911), China, pp, 54-65.

2. Zhenzi Wang, Dongmei Hou, Jiamei Hou (2002) “Fumigation Cosmetology” in “21st Century Medical University Teaching: Physical Therapy and Gymnastic Cosmetology” Zaien Hou, Jingwei Qi and Ming Guo, eds. Science Press China (ISBN: 7-03-010652-0/R.911), China, pp, 66-84.

3. Jingwei Qi, Dongmei Hou (2002) “Common Beauty of Fitness Training Methods” in “21st Century Medical University Teaching: Physical Therapy and Gymnastic Cosmetology” Zaien Hou, Jingwei Qi and Ming Guo, eds. Science Press China (ISBN: 7-03-010652-0/R.911), China, pp, 243-249.

4. Qun Ai, Dongmei Hou, Na Sun, Jie Bai, Zaien Hou (2002) “Acupuncture Cosmetology” in “21st Century Medical University Teaching: Meridian Cosmetology” Zaien Hou and Jingwei Qi, eds. Science Press China (ISBN: 7-03-010652-0/R.911), China, pp, 9-101.

5. Jingwei Qi, Yuzhen Hou, Qun Ai, Xia Zhao, Dongmei Hou (2002) “Massage Cosmetology” in “21st Century Medical University Teaching: Meridian Cosmetology” Zaien Hou and Jingwei Qi, eds. Science Press China (ISBN: 7-03-010652-0/R.911), China, pp, 103-157.

Conference Presentations

1. Dongmei Hou, Riccardo Olcese (2006)  A Functional Salt Bridge in BKCa Channel Outer Pore?  Biophysical Society, 2006 Annual Meeting, USA

2. Dongmei Hou, Riccardo Olcese (2006). Structural Diversity in K+ Channels Outer Pore. American Society of Anesthesiologists, 2006 Annual Meeting, USA

3. Dongmei Hou, Daniel Sigg, Riccardo Olcese (2006). Permeation properties of BK and Shaker K+ Channels. American Society of Anesthesiologists, 2006 Annual Meeting, USA

4. Dongmei Hou, Riccardo Olcese (2005) A Functional Salt Bridge in BK Channel Outer Pore? The Brain Research Institute at UCLA, Seventeenth Annual Neuroscience Poster Session, USA

5. Dongmei Hou, Kazuhiro Suzuki, Michael Forte, and Yoshiaki Kidokoro (2002)   Impairment of Synaptic Transmission in  Drosophila Embryos Lacking Gsα. The 25th Annual Meeting of Japanese Neuroscience Society, Japan   

6. Dongmei Hou, Hiroshi Kuromi, Yoshiaki Kidokoro (2000) Effects of Veratridine on the Presynaptic Function at Drosophila Neuromusculat Junctions. The 23rd Annual Meeting of Japanese Neuroscience Society, Japan   

7. Dongemi Hou, Jingwei Qi (1997) Livostin Treatment of Allergic Rhinitis Analysis. 5th National Otorhinolaryngology Clinic Progress Symposium on the New Compilation, China

8. Dongmei Hou, Jingwei Qi (1996) The Comprehensive Diagnosis and Treatment of the Serious Acute Epiglottitis.  The 4th Annual Meeting of Chinese Clinical Otorhinolaryngology Society, China

9. Dongmei Hou, Jingwei Qi (1995) Diagnosis and Treatment of Pediatric Laryngitis. The 3rd Annual Meeting of Chinese Clinical Otorhinolaryngology Society, China

10. Daniel Sigg, Dongmei Hou, Riccardo Olcese (2006).  Temperature and D2O as discriminators of permeation mechanisms in BKCa and Shaker Channels. Predictions of a 4-state Cyclic Model.  Biophysical Society, 2006 Annual Meeting, USA

11. Nicoletta Savalli, Dongmei Hou, Riccardo Olcese (2006). Ca2+ and Voltage Dependent Conformational Changes in BKCa Channel    Biophysical Society, 2006 Annual Meeting, USA

12. Nicoletta Savalli, Dongmei Hou, Daniel Sigg, Riccardo Olcese (2006). Ca2+ Dependent Conformational Changes in Human Large Conductance K+ Channels (BK) During Activation. American Society of Anesthesiologists, 2006 Annual Meeting, USA

13. Daniel Sigg, Dongmei Hou, Riccardo Olcese (2005)  Maximum Ion Throughput Across Small Free Energy Barriers in the Large-Conductance BKCa Channels: Evidence from Temperature and D2O Experiments. American Society of Anesthesiologists, 2005 Annual Meeting, USA

14. Daniel Sigg, Dongmei Hou, Riccardo Olcese (2005) Maximum Ion Throughput across Small Free Energy Barriers in the Large-Conductance BK Channels: Evidence from Temperature and D2O Experiments. The Brain Research Institute at UCLA, Seventeenth Annual Neuroscience Poster Session, USA

15. Jingwei Qi, Dongemi Hou (1997) Kang Nose in the Treatment of Rhinosinusitis Clinical Observation. 5th National Otorhinolaryngology Clinic Progress Symposium on the New Compilation, China

16. Taleh Yusifov, Nicoletta Savalli, Dongmei Hou, Nigar Yusifova, Riccardo Olcese (2007) Ca2+-Induced Conformational Changes in the RCK2 Domain of hSlo BKCa Channel
Biophysical Society, 2007 Annual Meeting, USA

17. Nicoletta Savalli, Taleh Yusifov, Dongmei Hou, Daniel Sigg, Riccardo Olcese (2007) Mutation in the “Ca2+ Bowl” abolishes the coupling between Ca2+ activation and S4 movement in BKCa channels. Biophysical Society, 2007 Annual Meeting, USA       

18. Roshni Vyas, Nicoletta Savalli, Dongmei Hou, Sarah Buxton, Riccardo Olcese (2005) Ca-Dependent Conformational Changes in BKCa channels.  The Brain Research Institute at UCLA, Seventeenth Annual Neuroscience Poster Session, USA

19. Masaki Sone, Emiko Suzuki, Mikio Hoshino, Hou Dong Mei,  Hiroshi Kuromi, Masaki Fukata, Shinya Kuroda, Kozo Kaibuchi, Yo-ichi Nabeshima, Chihiro Hama (1999) Localization and possible function of cell adhesion molecules and the Still life - Rac-pathway in the synapses.  Japanese Drosophila Research Conference 4th Workshop

Membership in Professional Associations

1. Member, Biophysical Society, 10/2005-present
2. Associate chairman of the executive council, International Cosmetology Medical Education Association (ICME), 7/2004-present
3. Member, Japanese Neuroscience Society, 1999-2003
4. Member, Chinese Medical Association, 1992-1998